Tailoring Hydrophobicity of Thioflavin T to Optimize Aβ Fibril Bioimaging

Fluorescence imaging of amyloid beta (Aβ) polypeptide using a small-molecule fluorophore is practical system for Alzheimer's disease (AD) diagnosis in clinical and research fields. Herein, molecular designing strategy new fluorogenic probe that equips bathochromic emission shift enhanced binding affinity toward Aβ fibril through structural optimization suggested. Thioflavin T (ThT)-derived fluorescent dyes are developed by adjusting the hydrophobicity electron donor moiety D–π–A structures with biannulated ring expansion. Indeed, probes tuned to have redshifted fluorescence wavelength optimized affinities fibrils vitro cortical imaging. Detection background signal minimized maintaining photophysical properties twisted intramolecular charge transfer (TICT), resulting significantly sensitive outstanding bioimaging AD.